Hotspot

rrn4.5-rrn23

IRa · 130533-130676 · 144 bp

Back to Chenopodiastrum

Pi 0.0928

MarkerSeek score 79.90

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 144
variable_sites: 9
pi: 0.0928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	191-238 bp	1.0	85.0
rrn4.5-rrn23_p2	CTGTGGGTCTGTTAGGATGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	225-272 bp	1.0	84.7
rrn4.5-rrn23_p3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	217-264 bp	1.0	84.6
rrn4.5-rrn23_p4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	219-266 bp	1.0	84.5
rrn4.5-rrn23_p5	CTGTGGGTCTGTTAGGATGC	TAGTAAGCCCACCCCAAGAT	55.0	50.0	58.0	57.7	222-269 bp	1.0	84.5