Hotspot

rpl32-trnL-UAG

SSC · 112491-113566 · 1076 bp

Back to Chenopodiastrum

Pi 0.079

MarkerSeek score 79.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1076
variable_sites: 83
pi: 0.079
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTATTGGGCGGCATTAAGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1209 bp	0.5	59.7
rpl32-trnL-UAG_p2	GGTATTGGGCGGCATTAAGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1210 bp	0.5	59.7
rpl32-trnL-UAG_p3	GGTATTGGGCGGCATTAAGA	GCATCATCAACCACTGGGAT	50.0	50.0	58.0	57.9	1813 bp	0.5	59.3
rpl32-trnL-UAG_p4	GGTATTGGGCGGCATTAAGA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	1230 bp	0.5	59.2
rpl32-trnL-UAG_p5	GGTATTGGGCGGCATTAAGA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.0	58.1	1232 bp	0.5	59.2