Hotspot

ndhF-rpl32

SSC · 111331-112316 · 986 bp

Back to Chenopodiastrum

Pi 0.0827

MarkerSeek score 82.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 986
variable_sites: 78
pi: 0.0827
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCATGCATATTGGTATATATGTTCCA	CGCTTTTTGTAAATCGAAGTACG	34.6	39.1	57.2	57.1	1054-1216 bp	1.0	48.5
ndhF-rpl32_p2	TCCATGCATATTGGTATATATGTTCC	CGCTTTTTGTAAATCGAAGTACG	34.6	39.1	57.0	57.1	1055-1217 bp	1.0	48.5
ndhF-rpl32_p3	CCATGCATATTGGTATATATGTTCCA	ACGAATACGCTTTTTGTAAATCGA	34.6	33.3	57.2	57.8	1061-1223 bp	1.0	48.5
ndhF-rpl32_p4	CCATGCATATTGGTATATATGTTCCA	ACGCTTTTTGTAAATCGAAGT	34.6	33.3	57.2	54.7	1055-1217 bp	1.0	48.5
ndhF-rpl32_p5	CCATGCATATTGGTATATATGTTCC	CGCTTTTTGTAAATCGAAGTACG	36.0	39.1	55.8	57.1	1054-1216 bp	1.0	48.5