Hotspot

rrn23-rrn4.5

IRb · 105725-105868 · 144 bp

Back to Chenopodiastrum

Pi 0.0928

MarkerSeek score 79.90

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 144
variable_sites: 9
pi: 0.0928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	50.0	50.0	58.2	58.0	191-238 bp	1.0	85.0
rrn23-rrn4.5_p2	AAGTAGTAAGCCCACCCCAA	CTGTGGGTCTGTTAGGATGC	50.0	55.0	58.2	58.0	225-272 bp	1.0	84.7
rrn23-rrn4.5_p3	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	50.0	55.0	58.2	58.0	217-264 bp	1.0	84.6
rrn23-rrn4.5_p4	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	50.0	55.0	58.2	58.1	219-266 bp	1.0	84.5
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	CTGTGGGTCTGTTAGGATGC	50.0	55.0	57.7	58.0	222-269 bp	1.0	84.5