Hotspot

trnP-UGG-psaJ

LSC · 66137-66569 · 433 bp

Back to Chenopodiastrum

Pi 0.0636

MarkerSeek score 77.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 26
pi: 0.0636
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	521-524 bp	1.0	84.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	520-523 bp	1.0	84.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	532-535 bp	1.0	84.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	531-534 bp	1.0	84.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	534-537 bp	1.0	84.4