Hotspot

psbE-petL

LSC · 64190-65281 · 1092 bp

Back to Chenopodiastrum

Pi 0.0723

MarkerSeek score 78.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 78
pi: 0.0723
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACTAGTTAGAGTAGACA	55.0	36.0	58.2	55.7	1140-1187 bp	1.0	46.3
psbE-petL_p2	TCTCCTGTGCTTCCAGACAT	CCGAAATAACTAGTTAGAGTAGACA	50.0	36.0	58.4	55.7	1138-1185 bp	1.0	46.3
psbE-petL_p3	TTCTCCTGTGCTTCCAGACA	CCGAAATAACTAGTTAGAGTAGACA	50.0	36.0	58.6	55.7	1139-1186 bp	1.0	46.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTAGTTAGAGTAGACA	50.0	36.0	57.8	55.7	1145-1192 bp	1.0	46.2
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	CCGAAATAACTAGTTAGAGTAGACA	45.0	36.0	57.4	55.7	1146-1193 bp	1.0	46.2