Hotspot

petA-psbJ

LSC · 62528-63390 · 863 bp

Back to Chenopodiastrum

Pi 0.0615

MarkerSeek score 78.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 53
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	CTGGATTAGGCTCATCGCTG	50.0	55.0	56.8	58.2	920-940 bp	1.0	73.7
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCG	TCTGGATTAGGCTCATCGCT	50.0	50.0	56.8	58.3	921-941 bp	1.0	73.0
petA-psbJ_p3	CGAGAAGGTTCAATTGTCCGA	CTGGATTAGGCTCATCGCTG	47.6	55.0	58.3	58.2	920-940 bp	1.0	72.5
petA-psbJ_p4	TCGAGAAGGTTCAATTGTCCG	CTGGATTAGGCTCATCGCTG	47.6	55.0	58.3	58.2	921-941 bp	1.0	72.5
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCGA	TCTGGATTAGGCTCATCGCT	47.6	50.0	58.3	58.3	921-941 bp	1.0	71.8