Hotspot

accD-psaI

LSC · 58561-59004 · 444 bp

Back to Chenopodiastrum

Pi 0.117

MarkerSeek score 79.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 46
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	AAAGAAGCCATTGCAATCGC	45.0	45.0	58.0	58.0	728-878 bp	1.0	89.2
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	AAAGAAGCCATTGCAATCGC	50.0	45.0	58.0	58.0	898-1048 bp	1.0	88.6
accD-psaI_p3	AACGCCTACATTGCATTTGC	CCCACATTCCACGAAATTGC	45.0	50.0	58.0	58.0	1364-1518 bp	1.0	87.7
accD-psaI_p4	AACGCCTACATTGCATTTGC	GGCCTGCAATCCCATAGAAA	45.0	50.0	58.0	57.9	1318-1472 bp	1.0	87.5
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	CCCACATTCCACGAAATTGC	50.0	50.0	58.0	58.0	1534-1688 bp	1.0	87.1