Hotspot

ndhC-rbcL

LSC · 50570-51303 · 734 bp

Back to Chenopodiastrum

Pi 0.0589

MarkerSeek score 81.00

Primer available yes

Label ndhC-rbcL

Feature Metadata

label_name: ndhC-rbcL
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 42
pi: 0.0589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-rbcL_p1	CGGGTCCTTTGTTAATCGGA	GCTGCTGCTTGTGAGATATG	50.0	50.0	57.6	57.0	903-912 bp	1.0	74.1
ndhC-rbcL_p2	TCGGGTCCTTTGTTAATCGG	GCTGCTGCTTGTGAGATATG	50.0	50.0	57.6	57.0	904-913 bp	1.0	74.1
ndhC-rbcL_p3	CGGGTCCTTTGTTAATCGGA	CTGCTGCTTGTGAGATATGGA	50.0	47.6	57.6	57.8	902-911 bp	1.0	72.9
ndhC-rbcL_p4	TCGGGTCCTTTGTTAATCGG	CTGCTGCTTGTGAGATATGGA	50.0	47.6	57.6	57.8	903-912 bp	1.0	72.9
ndhC-rbcL_p5	CGGGTCCTTTGTTAATCGGA	TGCTGCTTGTGAGATATGGA	50.0	45.0	57.6	56.6	901-910 bp	1.0	71.4