Hotspot

rps4-trnT-UGU

LSC · 46138-46522 · 385 bp

Back to Chenopodiastrum

Pi 0.0688

MarkerSeek score 77.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 26
pi: 0.0688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCTAGACGGCGTATTTTT	GATGGTCATCGGTTCGAGTC	50.0	55.0	57.7	58.2	466-504 bp	1.0	81.6
rps4-trnT-UGU_p2	CCCCTAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	497-535 bp	1.0	81.5
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	GATGGTCATCGGTTCGAGTC	55.0	55.0	58.3	58.2	512-550 bp	1.0	81.3
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	543-581 bp	1.0	81.2
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.3	57.5	551-589 bp	1.0	78.2