Hotspot

trnG-GCC-trnfM-CAU

LSC · 35964-36188 · 225 bp

Back to Chenopodiastrum

Pi 0.0648

MarkerSeek score 77.60

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 14
pi: 0.0648
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	298-303 bp	1.0	84.5
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	292-297 bp	1.0	84.0
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	334-339 bp	1.0	83.9
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	291-296 bp	1.0	83.5
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	296-301 bp	1.0	83.4