Hotspot

trnS-GCU-trnG-UCC

LSC · 7847-8572 · 726 bp

Back to Chenopodiastrum

Pi 0.0862

MarkerSeek score 79.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 62
pi: 0.0862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	835-912 bp	1.0	53.0
trnS-GCU-trnG-UCC_p2	CGAATGACTCGTACAACGGA	TACCACTAAACTATACCCGC	50.0	45.0	57.7	54.0	805-882 bp	1.0	52.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	836-913 bp	1.0	51.9
trnS-GCU-trnG-UCC_p4	CGAATGACTCGTACAACGGA	TTACCACTAAACTATACCCGC	50.0	42.9	57.7	54.9	806-883 bp	1.0	51.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TTTACCACTAAACTATACCCGC	50.0	40.9	58.2	55.6	837-914 bp	1.0	50.2