Hotspot

psbI-trnS-GCU

LSC · 7560-7758 · 199 bp

Back to Chenopodiastrum

Pi 0.0909

MarkerSeek score 82.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 18
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGACGCGAAGAA	GTCATTCGTACCGAGGGTTC	50.0	55.0	57.7	58.1	259-260 bp	1.0	82.9
psbI-trnS-GCU_p2	GTAATCCTGGACGCGAAGAA	AGTCATTCGTACCGAGGGTT	50.0	50.0	57.7	58.4	260-261 bp	1.0	80.0
psbI-trnS-GCU_p3	GTAATCCTGGACGCGAAGAA	TTTTCAACGGGGAGAGATGG	50.0	50.0	57.7	57.5	320-321 bp	1.0	78.5
psbI-trnS-GCU_p4	GTAATCCTGGACGCGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.7	58.6	305-306 bp	1.0	77.6
psbI-trnS-GCU_p5	GTAATCCTGGACGCGAAGAA	GAGTCATTCGTACCGAGGGT	50.0	55.0	57.7	58.9	261-262 bp	1.0	76.3