Hotspot

ycf1

SSC · 128008-133164 · 5157 bp

Back to Cheniella

Pi 0.0042

MarkerSeek score 44.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5157
variable_sites: 89
pi: 0.0042
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCCACGAGAAGATCCCTT	GCTGGGGTTATGGAAGAAGG	50.0	55.0	58.1	57.9	1244 bp	0.066667	41.7
ycf1_p2	TTGTCCACGAGAAGATCCCT	GCTGGGGTTATGGAAGAAGG	50.0	55.0	58.1	57.9	1245 bp	0.066667	41.7
ycf1_p3	TGTCCACGAGAAGATCCCTT	TAGCAGCAACAACTGGGTTT	50.0	45.0	58.1	57.9	1210 bp	0.066667	41.6
ycf1_p4	TTGTCCACGAGAAGATCCCT	TAGCAGCAACAACTGGGTTT	50.0	45.0	58.1	57.9	1211 bp	0.066667	41.6
ycf1_p5	TGTCCACGAGAAGATCCCTT	CTTCTGCAATAGGCCCCAAT	50.0	50.0	58.1	57.9	1077 bp	0.066667	41.6