Hotspot

ndhF

SSC · 113869-116112 · 2244 bp

Back to Cheniella

Pi 0.00129

MarkerSeek score 32.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 16
pi: 0.00129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCTGGGGTTATGGAAGAAGG	TTTCACTTCGTGCTTTGCAA	55.0	40.0	57.9	57.1	2807-2949 bp	1.0	75.6
ndhF_p2	GCTGGGGTTATGGAAGAAGG	TTTTCACTTCGTGCTTTGCA	55.0	40.0	57.9	57.1	2808-2950 bp	1.0	75.6
ndhF_p3	TAGCAGCAACAACTGGGTTT	TTTCACTTCGTGCTTTGCAA	45.0	40.0	57.9	57.1	2773-2915 bp	1.0	75.6
ndhF_p4	TAGCAGCAACAACTGGGTTT	TTTTCACTTCGTGCTTTGCA	45.0	40.0	57.9	57.1	2774-2916 bp	1.0	75.6
ndhF_p5	CTTCTGCAATAGGCCCCAAT	TTTCACTTCGTGCTTTGCAA	50.0	40.0	57.9	57.1	2640-2782 bp	1.0	75.6