Hotspot

clpP

LSC · 72660-74709 · 2050 bp

Back to Cheniella

Pi 0.00362

MarkerSeek score 61.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2050
variable_sites: 41
pi: 0.00362
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GACGATCAAACGCAAACAGT	TCGTTACGCTTCTGCATCAA	45.0	45.0	57.3	57.9	2247-2304 bp	1.0	78.7
clpP_p2	GACGATCAAACGCAAACAGT	ACGCTTCTGCATCAAAGTCA	45.0	45.0	57.3	58.1	2242-2299 bp	1.0	78.4
clpP_p3	GACGATCAAACGCAAACAGT	AACCCATCGTTACGCTTCTG	45.0	50.0	57.3	58.3	2253-2310 bp	1.0	77.1
clpP_p4	GACGATCAAACGCAAACAGT	ATCGTTACGCTTCTGCATCA	45.0	45.0	57.3	57.7	2248-2305 bp	1.0	76.9
clpP_p5	AGACGATCAAACGCAAACAG	TCGTTACGCTTCTGCATCAA	45.0	45.0	57.1	57.9	2248-2305 bp	1.0	76.4