Hotspot

ndhG-ndhI

SSC · 122538-123056 · 519 bp

Back to Cheniella

Pi 0.00738

MarkerSeek score 67.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 519
variable_sites: 17
pi: 0.00738
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	637-678 bp	1.0	84.9
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	700-741 bp	1.0	84.6
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	TTTGGGCCGTTTACCAATGT	50.0	45.0	58.0	58.3	626-667 bp	1.0	83.4
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAA	TTTGGGCCGTTTACCAATGT	45.0	45.0	57.9	58.3	689-730 bp	1.0	83.1
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	CTTTGGGCCGTTTACCAATG	50.0	50.0	58.0	57.6	627-668 bp	1.0	82.8