Hotspot

rpl32-trnL-UAG

SSC · 117327-117890 · 564 bp

Back to Cheniella

Pi 0.0127

MarkerSeek score 75.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 564
variable_sites: 27
pi: 0.0127
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	497-714 bp	1.0	87.1
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	498-715 bp	1.0	87.1
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	498-715 bp	1.0	87.0
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	499-716 bp	1.0	87.0
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	507-724 bp	1.0	86.1