Hotspot

ycf1-ndhF

SSC · 113753-113868 · 116 bp

Back to Cheniella

Pi 0.0857

MarkerSeek score 68.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 31
pi: 0.0857
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCTGGGGTTATGGAAGAAGG	ACTTTCTTCGTTGGCACCAT	55.0	45.0	57.9	58.0	912-1059 bp	1.0	86.6
ycf1-ndhF_p2	TAGCAGCAACAACTGGGTTT	ACTTTCTTCGTTGGCACCAT	45.0	45.0	57.9	58.0	878-1025 bp	1.0	86.6
ycf1-ndhF_p3	TAGCAGCAACAACTGGGTTT	AAAACCTAACGCGACCCTTT	45.0	45.0	57.9	58.0	994-1141 bp	1.0	86.1
ycf1-ndhF_p4	GCTGGGGTTATGGAAGAAGG	AAAAACCTAACGCGACCCTT	55.0	45.0	57.9	58.0	1029-1176 bp	1.0	86.0
ycf1-ndhF_p5	GCTGGGGTTATGGAAGAAGG	AAAACCTAACGCGACCCTTT	55.0	45.0	57.9	58.0	1028-1175 bp	1.0	86.0