Hotspot

rps3-rps19

LSC · 86606-87561 · 956 bp

Back to Cheniella

Pi 0.00269

MarkerSeek score 58.70

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 15
pi: 0.00269
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	GCCTACTCGAAACTTCCTGG	50.0	55.0	57.5	58.0	1042-1054 bp	1.0	79.3
rps3-rps19_p2	TACCAAGTCTGAAACCGAGC	AACTTCCTGGGACATGATGC	50.0	50.0	57.5	57.9	1032-1044 bp	1.0	78.3
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	TTCGCCTACTCGAAACTTCC	50.0	50.0	57.5	57.6	1045-1057 bp	1.0	76.5
rps3-rps19_p4	TACCAAGTCTGAAACCGAGC	CGCCTACTCGAAACTTCCTG	50.0	55.0	57.5	58.4	1043-1055 bp	1.0	76.4
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	TCGCCTACTCGAAACTTCCT	50.0	50.0	57.5	58.5	1044-1056 bp	1.0	75.7