Hotspot

psbE-petL

LSC · 66875-68200 · 1326 bp

Back to Cheniella

Pi 0.00412

MarkerSeek score 60.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1326
variable_sites: 18
pi: 0.00412
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGTTCAGACCGA	45.0	47.6	58.0	57.5	1519-1533 bp	1.0	70.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAGACCGA	50.0	47.6	57.8	57.5	1431-1445 bp	1.0	69.2
psbE-petL_p3	CCCGCAATGAATAGAGAGGG	GTCGTATCTTGTTCAGACCGA	55.0	47.6	57.8	57.5	1505-1519 bp	1.0	68.9
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGTCGTATCTTGTTCAGACCG	45.0	47.6	58.0	57.5	1520-1534 bp	0.933333	67.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGTTCAGACCG	50.0	47.6	57.8	57.5	1432-1446 bp	0.933333	65.9