Hotspot

rps16-trnQ-UUG

LSC · 6756-7396 · 641 bp

Back to Cheniella

Pi 0.00557

MarkerSeek score 62.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 13
pi: 0.00557
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCACACGTTGCTTTCTACCC	AAGGTTCGAATCCTTCCGTC	50.0	50.0	58.1	57.6	655-728 bp	1.0	81.4
rps16-trnQ-UUG_p2	TCACACGTTGCTTTCTACCC	TTGGTCCCGTTATTCGAAGG	50.0	50.0	58.1	57.6	671-744 bp	1.0	81.3
rps16-trnQ-UUG_p3	TCACACGTTGCTTTCTACCC	GGTCCCGTTATTCGAAGGTT	50.0	50.0	58.1	57.6	669-742 bp	1.0	81.3
rps16-trnQ-UUG_p4	ACACGTTGCTTTCTACCCAA	AAGGTTCGAATCCTTCCGTC	45.0	50.0	57.7	57.6	653-726 bp	1.0	79.7
rps16-trnQ-UUG_p5	ACACGTTGCTTTCTACCCAA	TTGGTCCCGTTATTCGAAGG	45.0	50.0	57.7	57.6	669-742 bp	1.0	79.6