Hotspot

ycf3

LSC · 44019-45982 · 1964 bp

Back to Chengiodendron

Pi 0

MarkerSeek score 57.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1964
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATGGGGCCAACAAACAAT	TCCCACGAGAGGTAATGAGA	45.0	50.0	58.3	57.2	2093-2094 bp	1.0	71.3
ycf3_p2	ATTTGACTGATGGGGCCAAC	TCCCACGAGAGGTAATGAGA	50.0	50.0	58.4	57.2	2100-2101 bp	1.0	70.0
ycf3_p3	TGATGGGGCCAACAAACAAT	TCCCACGAGAGGTAATGAGAA	45.0	47.6	58.3	57.9	2093-2094 bp	1.0	69.1
ycf3_p4	TGATGGGGCCAACAAACAAT	TTCCCACGAGAGGTAATGAGA	45.0	47.6	58.3	57.9	2094-2095 bp	1.0	69.1
ycf3_p5	ATTTGACTGATGGGGCCAAC	TCCCACGAGAGGTAATGAGAA	50.0	47.6	58.4	57.9	2100-2101 bp	1.0	67.8