Hotspot

rps16

LSC · 5278-6421 · 1144 bp

Back to Chengiodendron

Pi 0.000874

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1144
variable_sites: 1
pi: 0.000874
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCGACAGAAGCAATGAGAAA	TCAATTCCATACCGGTTCCA	40.0	45.0	55.0	56.5	1298 bp	1.0	44.5
rps16_p2	TTCGACAGAAGCAATGAGAA	TCAATTCCATACCGGTTCCA	40.0	45.0	55.0	56.5	1299 bp	1.0	44.5
rps16_p3	CGAATAAAGCAGGATTAAGGGG	TCAATTCCATACCGGTTCCA	45.5	45.0	56.9	56.5	1272 bp	1.0	43.4
rps16_p4	TTCGACAGAAGCAATGAGAAA	TCAATTCCATACCGGTTCCA	38.1	45.0	55.8	56.5	1299 bp	1.0	42.9
rps16_p5	TCGACAGAAGCAATGAGAAA	TCAATTCCATACCGGTTCCAA	40.0	42.9	55.0	57.3	1298 bp	1.0	42.5