Hotspot

psbD

LSC · 34326-35387 · 1062 bp

Back to Chengiodendron

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	CATTGGAAGGGGCAGTGTAA	AATAAGTCGAGCATTCCCGG	50.0	50.0	57.8	57.8	1252 bp	1.0	76.4
psbD_p2	GCTCGGAAATGGTCGAAGTA	AATAAGTCGAGCATTCCCGG	50.0	50.0	57.7	57.8	1177 bp	1.0	75.8
psbD_p3	AAATACTGTGAGGTGCTCGG	AATAAGTCGAGCATTCCCGG	50.0	50.0	57.6	57.8	1191 bp	1.0	75.0
psbD_p4	AATACTGTGAGGTGCTCGGA	AATAAGTCGAGCATTCCCGG	50.0	50.0	58.4	57.8	1190 bp	1.0	74.6
psbD_p5	ATACTGTGAGGTGCTCGGAA	AATAAGTCGAGCATTCCCGG	50.0	50.0	58.4	57.8	1189 bp	1.0	74.6