Hotspot

petD

LSC · 78838-80052 · 1215 bp

Back to Chengiodendron

Pi 0.000823

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1215
variable_sites: 1
pi: 0.000823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCCCTTCAACCAATCATGTCA	TCCCTAGATACACACGTCGT	42.9	50.0	57.2	57.6	1323 bp	1.0	62.4
petD_p2	TGAGGGGAATTCTACGAAGAGA	TCCCTAGATACACACGTCGT	45.5	50.0	58.0	57.6	1288 bp	1.0	60.6
petD_p3	TCCCTTCAACCAATCATGTCAA	TCCCTAGATACACACGTCGT	40.9	50.0	57.9	57.6	1323 bp	1.0	59.9
petD_p4	TTCCCTTCAACCAATCATGTCA	TCCCTAGATACACACGTCGT	40.9	50.0	57.9	57.6	1324 bp	1.0	59.9
petD_p5	GAGTTGAGGGGAATTCTACGA	TCCCTAGATACACACGTCGT	47.6	50.0	56.8	57.6	1292 bp	1.0	59.5