Hotspot

petB

LSC · 77302-78655 · 1354 bp

Back to Chengiodendron

Pi 0.000739

MarkerSeek score 59.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1354
variable_sites: 1
pi: 0.000739
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AACCGCAAAGTCCTGTCTTT	TCCTCCTCCAAGTGATAAATGA	45.0	40.9	57.9	56.3	1522 bp	1.0	49.6
petB_p2	ACCGCAAAGTCCTGTCTTTT	TCCTCCTCCAAGTGATAAATGA	45.0	40.9	57.9	56.3	1521 bp	1.0	49.6
petB_p3	GCTCGGATTTCCAGCCTATT	TCCTCCTCCAAGTGATAAATGA	50.0	40.9	57.7	56.3	1477 bp	1.0	48.0
petB_p4	AACCGCAAAGTCCTGTCTTT	TTCCTCCTCCAAGTGATAAATGA	45.0	39.1	57.9	57.0	1523 bp	1.0	47.3
petB_p5	ACCGCAAAGTCCTGTCTTTT	TTCCTCCTCCAAGTGATAAATGA	45.0	39.1	57.9	57.0	1522 bp	1.0	47.3