Hotspot

ndhD

SSC · 117164-118666 · 1503 bp

Back to Chengiodendron

Pi 0.000665

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTTCATTGTACAACGAACGA	GGTTTTCTGGCCCAAGTGTA	35.0	50.0	53.5	58.0	1614-1615 bp	1.0	44.3
ndhD_p2	TTTTCATTGTACAACGAACGA	GGTTTTCTGGCCCAAGTGTA	33.3	50.0	54.4	58.0	1615-1616 bp	1.0	43.3
ndhD_p3	TTTCATTGTACAACGAACGA	TTTTGAGCACGGGGTTTTCT	35.0	45.0	53.5	58.2	1626-1627 bp	1.0	42.6
ndhD_p4	TCATTGTACAACGAACGATT	GGTTTTCTGGCCCAAGTGTA	35.0	50.0	53.3	58.0	1612-1613 bp	1.0	42.4
ndhD_p5	TTCATTGTACAACGAACGAT	GGTTTTCTGGCCCAAGTGTA	35.0	50.0	53.3	58.0	1613-1614 bp	1.0	42.4