Hotspot

matK

LSC · 2123-3643 · 1521 bp

Back to Chengiodendron

Pi 0.000657

MarkerSeek score 59.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 1
pi: 0.000657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATCAACATTTCAGAATAGTGGA	AGTTTACCTGTTTCCGAGGT	33.3	45.0	56.4	56.4	1739 bp	1.0	40.0
matK_p2	ACATCAACATTTCAGAATAGTGGA	AAGTTTACCTGTTTCCGAGGT	33.3	42.9	56.4	57.1	1740 bp	1.0	40.0
matK_p3	ACATCAACATTTCAGAATAGTGGAA	AGTTTACCTGTTTCCGAGGT	32.0	45.0	57.0	56.4	1739 bp	1.0	40.0
matK_p4	ACATCAACATTTCAGAATAGTGG	AGTTTACCTGTTTCCGAGGT	34.8	45.0	55.0	56.4	1739 bp	1.0	40.0
matK_p5	ACATCAACATTTCAGAATAGTGGA	CCTTGTTTTGACTGTATCGCA	33.3	42.9	56.4	56.8	1700 bp	1.0	40.0