Hotspot

clpP

LSC · 72338-74385 · 2048 bp

Back to Chengiodendron

Pi 0.000489

MarkerSeek score 57.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2048
variable_sites: 1
pi: 0.000489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGCTGGTTTAGATTGATCC	TGTTGTTACGTTTCCACATCA	47.6	38.1	57.7	56.0	2222-2223 bp	1.0	46.0
clpP_p2	TGGGCTGGTTTAGATTGATCC	TGTTGTTACGTTTCCACATCAA	47.6	36.4	57.7	56.8	2222-2223 bp	1.0	43.9
clpP_p3	GGGCTGGTTTAGATTGATCCT	TGTTGTTACGTTTCCACATCA	47.6	38.1	57.4	56.0	2221-2222 bp	1.0	43.7
clpP_p4	TGGGCTGGTTTAGATTGATCC	ATGTTGTTACGTTTCCACATCA	47.6	36.4	57.7	56.6	2223-2224 bp	1.0	42.2
clpP_p5	TGGGCTGGTTTAGATTGATCC	TGTTACGTTTCCACATCAAAGT	47.6	36.4	57.7	56.5	2219-2220 bp	1.0	41.7