Hotspot

atpF

LSC · 12728-13979 · 1252 bp

Back to Chengiodendron

Pi 0.000799

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1252
variable_sites: 1
pi: 0.000799
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGGCAAAGTGATACAAAAAGAACT	50.0	33.3	57.7	57.3	1395 bp	1.0	40.1
atpF_p2	GGCTCGAATGGTTACCATGA	ACTCTGATCGATTTTTGAGTCT	50.0	36.4	57.7	55.2	1374 bp	1.0	40.1
atpF_p3	GGCTCGAATGGTTACCATGA	AGAACTCTGATCGATTTTTGAGTC	50.0	37.5	57.7	57.0	1377 bp	1.0	40.1
atpF_p4	GGCTCGAATGGTTACCATGA	GAACTCTGATCGATTTTTGAGTCT	50.0	37.5	57.7	57.0	1376 bp	1.0	40.1
atpF_p5	GGCTCGAATGGTTACCATGA	GGCAAAGTGATACAAAAAGAACTC	50.0	37.5	57.7	57.0	1394 bp	1.0	40.1