Hotspot

rbcL-accD

LSC · 58517-59185 · 669 bp

Back to Chengiodendron

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 669
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACCTGCGTTCTAACTCCTTC	55.0	50.0	58.0	57.3	853 bp	1.0	73.8
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ACCTGCGTTCTAACTCCTTC	50.0	50.0	57.9	57.3	823 bp	1.0	73.2
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACCTGCGTTCTAACTCCTTC	55.0	50.0	57.7	57.3	839 bp	1.0	71.8
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	ACCTGCGTTCTAACTCCTTC	55.0	50.0	58.3	57.3	851 bp	1.0	71.5
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	CCTGCGTTCTAACTCCTTCT	55.0	50.0	58.0	57.0	852 bp	1.0	71.5