Hotspot

trnC(GCA)-petN

LSC · 29175-29948 · 774 bp

Back to Chengiodendron

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label trnC(GCA)-petN

Feature Metadata

label_name: trnC(GCA)-petN
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(GCA)-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	884 bp	1.0	79.4
trnC(GCA)-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	882 bp	1.0	79.4
trnC(GCA)-petN_p3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	936 bp	1.0	78.9
trnC(GCA)-petN_p4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	934 bp	1.0	78.9
trnC(GCA)-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	885 bp	1.0	78.8