Hotspot

rps16-trnQ(UUG)

LSC · 6422-8003 · 1582 bp

Back to Chengiodendron

Pi 0.000632

MarkerSeek score 58.90

Primer available yes

Label rps16-trnQ(UUG)

Feature Metadata

label_name: rps16-trnQ(UUG)
feature_type: igs
region: LSC
length_bp: 1582
variable_sites: 1
pi: 0.000632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	1669 bp	1.0	78.8
rps16-trnQ(UUG)_p2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1666 bp	1.0	78.8
rps16-trnQ(UUG)_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1655 bp	1.0	78.3
rps16-trnQ(UUG)_p4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	1675 bp	1.0	77.3
rps16-trnQ(UUG)_p5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1668 bp	1.0	76.6