Hotspot

ycf1

SSC · 116999-122620 · 5622 bp

Back to Cheirostylis

Pi 0.0423

MarkerSeek score 71.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5622
variable_sites: 537
pi: 0.0423
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGATTGCGACGAAAATCCGA	TCAACGGAGCTTATCGAGGA	45.0	50.0	58.0	58.2	553-589 bp	1.0	89.9
ycf1_p2	CCGATTGTTGGGAGTAACGT	TCAACGGAGCTTATCGAGGA	50.0	50.0	57.9	58.2	537-573 bp	1.0	89.4
ycf1_p3	AGATTGCGACGAAAATCCGA	CAACGGAGCTTATCGAGGAA	45.0	50.0	58.0	57.4	552-588 bp	1.0	87.2
ycf1_p4	AAGCGGGGAATGTGCATTTA	TCAACGGAGCTTATCGAGGA	45.0	50.0	58.2	58.2	647-668 bp	0.8	75.1
ycf1_p5	TACCACTCGGGGGATTGTAA	TCAACGGAGCTTATCGAGGA	50.0	50.0	57.7	58.2	979-1006 bp	0.4	55.9