Hotspot

clpP

LSC · 66488-68685 · 2198 bp

Back to Cheirostylis

Pi 0.0649

MarkerSeek score 83.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2198
variable_sites: 316
pi: 0.0649
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGTCGCACATACACCCTAGT	ACGCAATGGGAGAGTTTTGT	50.0	45.0	58.2	58.0	2700-2945 bp	0.8	82.6
clpP_p2	TGGCTGTCTTGCGTTTCTAA	ACGCAATGGGAGAGTTTTGT	45.0	45.0	57.8	58.0	2620-2865 bp	0.8	81.9
clpP_p3	TTGGCTGTCTTGCGTTTCTA	ACGCAATGGGAGAGTTTTGT	45.0	45.0	57.8	58.0	2621-2866 bp	0.8	81.9
clpP_p4	TGGTTGGCATGTGATGTCTT	ACGCAATGGGAGAGTTTTGT	45.0	45.0	57.7	58.0	2588-2833 bp	0.8	81.5
clpP_p5	ATTGGCTGTCTTGCGTTTCT	ACGCAATGGGAGAGTTTTGT	45.0	45.0	58.4	58.0	2622-2867 bp	0.8	80.7