Hotspot

accD

LSC · 54757-56289 · 1533 bp

Back to Cheirostylis

Pi 0.0695

MarkerSeek score 83.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 197
pi: 0.0695
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGGAATGGCGGATCATTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	2631-2731 bp	0.8	81.9
accD_p2	TTGGGAATGGCGGATCATTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	2632-2732 bp	0.8	81.9
accD_p3	TGGGAATGGCGGATCATTTT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.8	57.8	2621-2721 bp	0.8	81.2
accD_p4	TTGGGAATGGCGGATCATTT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.8	57.8	2622-2722 bp	0.8	81.2
accD_p5	CTATGGCTCCTTGGGAATGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	57.7	57.9	2642-2697 bp	0.4	57.6