Hotspot

ndhF-rpl32

SSC · 108810-109330 · 521 bp

Back to Cheirostylis

Pi 0.0673

MarkerSeek score 77.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 521
variable_sites: 56
pi: 0.0673
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	615-1281 bp	1.0	91.7
ndhF-rpl32_p2	ACACATTCCCACAAGTTCCC	AATCTGTTTCCACCGGACAG	50.0	50.0	58.0	57.8	623-1289 bp	1.0	90.5
ndhF-rpl32_p3	AAGTGGATCGATCAAGTGCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	420-857 bp	0.6	70.0
ndhF-rpl32_p4	CATTCCCACAAGTTCCCCAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	1049 bp	0.2	47.6
ndhF-rpl32_p5	CATTCCCACAAGTTCCCCAA	AATCTGTTTCCACCGGACAG	50.0	50.0	58.0	57.8	1057 bp	0.2	46.5