Hotspot

petA-psbJ

LSC · 60482-61110 · 629 bp

Back to Cheirostylis

Pi 0.0489
MarkerSeek score 86.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
629
variable_sites
69
pi
0.0489
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAAAAGGTTCAGTTGTACGA TATTCCGGGTTGGGTTCATC 38.1 50.0 55.4 57.3 690-1038 bp 1.0 54.7
petA-psbJ_p2 TGAAAAGGTTCAGTTGTACGA CTATTCCGGGTTGGGTTCAT 38.1 50.0 55.4 57.3 691-1039 bp 1.0 54.6
petA-psbJ_p3 ACAGTTTGAAAAGGTTCAGTTGT TATTCCGGGTTGGGTTCATC 34.8 50.0 57.3 57.3 696-1044 bp 1.0 53.5
petA-psbJ_p4 ACAGTTTGAAAAGGTTCAGTTGT CTATTCCGGGTTGGGTTCAT 34.8 50.0 57.3 57.3 697-1045 bp 1.0 53.5
petA-psbJ_p5 AGGTTCAGTTGTACGAAATGA TATTCCGGGTTGGGTTCATC 38.1 50.0 55.2 57.3 685-1033 bp 1.0 52.9