Hotspot

ycf4-cemA

LSC · 57870-58575 · 706 bp

Back to Cheirostylis

Pi 0.0482

MarkerSeek score 83.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 76
pi: 0.0482
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCGTCGCATCTTTCTTCGTT	AAGGATTTTGGATTGCCCGT	45.0	45.0	57.9	58.1	1048-1074 bp	0.8	76.9
ycf4-cemA_p2	TCGTCGCATCTTTCTTCGTT	AGGATTTTGGATTGCCCGTT	45.0	45.0	57.9	58.1	1047-1073 bp	0.8	76.9
ycf4-cemA_p3	ATCAGAATGGAGGCGAGAGA	AGGATTTTGGATTGCCCGTT	50.0	45.0	57.9	58.1	1004-1030 bp	0.8	76.9
ycf4-cemA_p4	TATGGGATCGCGGGTCTATT	AAGGATTTTGGATTGCCCGT	50.0	45.0	58.1	58.1	1182-1208 bp	0.8	76.4
ycf4-cemA_p5	TATGGGATCGCGGGTCTATT	AGGATTTTGGATTGCCCGTT	50.0	45.0	58.1	58.1	1181-1207 bp	0.8	76.4