Hotspot

ndhC-trnV_UAC

LSC · 48172-48808 · 637 bp

Back to Cheirostylis

Pi 0.0509

MarkerSeek score 85.20

Primer available yes

Label ndhC-trnV_UAC

Feature Metadata

label_name: ndhC-trnV_UAC
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 70
pi: 0.0509
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_UAC_p1	ACCATTCCAACGCTCCTTTT	TGCTTTTCAAGGGAGCCAAT	45.0	45.0	58.0	58.0	1584-1637 bp	1.0	89.3
ndhC-trnV_UAC_p2	ACCATTCCAACGCTCCTTTT	AATGCTTTTCAAGGGAGCCA	45.0	45.0	58.0	58.0	1586-1639 bp	1.0	89.2
ndhC-trnV_UAC_p3	ACCATTCCAACGCTCCTTTT	ATGCTTTTCAAGGGAGCCAA	45.0	45.0	58.0	58.0	1585-1638 bp	1.0	89.2
ndhC-trnV_UAC_p4	TGGTTCAGGCATTTGCTCAT	TGCTTTTCAAGGGAGCCAAT	45.0	45.0	58.1	58.0	1942-1995 bp	1.0	87.8
ndhC-trnV_UAC_p5	ACCATTCCAACGCTCCTTTT	AGGTGCAAATTATGGTGCCA	45.0	45.0	58.0	58.1	1487-1516 bp	0.4	58.0