Hotspot

trnL_UAA-trnF_GAA

LSC · 45413-45847 · 435 bp

Back to Cheirostylis

Pi 0.0701

MarkerSeek score 85.90

Primer available yes

Label trnL_UAA-trnF_GAA

Feature Metadata

label_name: trnL_UAA-trnF_GAA
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 65
pi: 0.0701
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL_UAA-trnF_GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	503-526 bp	1.0	87.8
trnL_UAA-trnF_GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	465-488 bp	1.0	84.6
trnL_UAA-trnF_GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	500-523 bp	1.0	84.5
trnL_UAA-trnF_GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	492-515 bp	1.0	83.9
trnL_UAA-trnF_GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	475-498 bp	1.0	83.4