Hotspot

atpH-atpI

LSC · 12417-12915 · 499 bp

Back to Cheirostylis

Pi 0.0406

MarkerSeek score 85.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 47
pi: 0.0406
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	589-615 bp	1.0	87.7
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	588-614 bp	1.0	87.2
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.3	57.9	583-609 bp	1.0	86.9
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.9	58.3	583-609 bp	1.0	86.6
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	50.0	55.0	57.8	58.3	582-608 bp	1.0	86.1