Hotspot

trnK_UUU-rps16

LSC · 4217-4720 · 504 bp

Back to Cheirostylis

Pi 0.0862

MarkerSeek score 81.80

Primer available yes

Label trnK_UUU-rps16

Feature Metadata

label_name: trnK_UUU-rps16
feature_type: igs
region: LSC
length_bp: 504
variable_sites: 89
pi: 0.0862
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK_UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	647-829 bp	1.0	89.5
trnK_UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	646-828 bp	1.0	89.5
trnK_UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.0	57.9	738-809 bp	0.8	80.9
trnK_UUU-rps16_p4	TCAGGATCAGTCGTGGTCTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	734-805 bp	0.8	80.5
trnK_UUU-rps16_p5	TTCAGGATCAGTCGTGGTCT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.1	57.9	735-806 bp	0.8	80.5