Hotspot

ycf1

SSC · 108100-113304 · 5205 bp

Back to Cheirolophus

Pi 0.00403

MarkerSeek score 54.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 21
pi: 0.00403
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCACAGGCGGAAGAAGAAA	GAAAACAACTTTCGGCGTCG	45.0	50.0	57.4	58.6	1321 bp	1.0	70.6
ycf1_p2	AAAAGCAAGGCCAGGACTAA	GAAAACAACTTTCGGCGTCG	45.0	50.0	57.3	58.6	1234 bp	1.0	70.2
ycf1_p3	AAAGCAAGGCCAGGACTAAA	GAAAACAACTTTCGGCGTCG	45.0	50.0	57.3	58.6	1233 bp	1.0	70.2
ycf1_p4	AAGCAAGGCCAGGACTAAAA	GAAAACAACTTTCGGCGTCG	45.0	50.0	57.3	58.6	1232 bp	1.0	70.2
ycf1_p5	AGCAAGGCCAGGACTAAAAA	GAAAACAACTTTCGGCGTCG	45.0	50.0	57.3	58.6	1231 bp	1.0	70.2