Hotspot

cemA

LSC · 60599-61288 · 690 bp

Back to Cheirolophus

Pi 0.0058

MarkerSeek score 62.10

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 4
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAGTCGACGAATGAGATGGG	GAATCTCCGGGATGGGTAGA	55.0	55.0	57.9	58.0	851 bp	1.0	79.3
cemA_p2	CGACGAATGAGATGGGTTCA	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.7	58.0	847 bp	1.0	77.9
cemA_p3	GTCGACGAATGAGATGGGTT	GAATCTCCGGGATGGGTAGA	50.0	55.0	57.7	58.0	849 bp	1.0	77.9
cemA_p4	GAGTCGACGAATGAGATGGG	CTCCGGGATGGGTAGAAAGA	55.0	55.0	57.9	58.2	847 bp	1.0	77.7
cemA_p5	GAGTCGACGAATGAGATGGG	TCTCCGGGATGGGTAGAAAG	55.0	55.0	57.9	58.2	848 bp	1.0	77.7