Hotspot

ccsA

SSC · 121746-122714 · 969 bp

Back to Cheirolophus

Pi 0.00206

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAACCGCGTGAATCACTAC	ATTAATTTAGGGGATTCTCTCC	50.0	36.4	58.0	52.7	1121 bp	1.0	40.1
ccsA_p2	AGAACCGCGTGAATCACTAC	AATTAATTTAGGGGATTCTCTCC	50.0	34.8	58.0	53.6	1122 bp	1.0	40.1
ccsA_p3	TGATTCACATGGTTCTCGCC	ATTAATTTAGGGGATTCTCTCC	50.0	36.4	58.3	52.7	1095 bp	1.0	40.1
ccsA_p4	GAGAACCGCGTGAATCACTA	ATTAATTTAGGGGATTCTCTCC	50.0	36.4	57.7	52.7	1122 bp	1.0	40.1
ccsA_p5	GAACCGCGTGAATCACTACA	ATTAATTTAGGGGATTCTCTCC	50.0	36.4	58.3	52.7	1120 bp	1.0	40.1