Hotspot

ycf1

SSC · 126571-132219 · 5649 bp

Back to Cheirodendron

Pi 0.00196

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5649
variable_sites: 11
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTAATCAGTATCAGGAAGAAGG	TTGAGGCTCTAGATATGGGA	41.7	45.0	57.5	54.4	250 bp	1.0	40.6
ycf1_p2	GGTAATCAGTATCAGGAAGAAGGA	TTGAGGCTCTAGATATGGGA	41.7	45.0	57.3	54.4	249 bp	1.0	40.6
ycf1_p3	TGGTAATCAGTATCAGGAAGAAGG	TGAGGCTCTAGATATGGGAT	41.7	45.0	57.5	54.1	249 bp	1.0	40.6
ycf1_p4	ACTCTTGGTAATCAGTATCAGGA	TTGAGGCTCTAGATATGGGA	39.1	45.0	56.2	54.4	255 bp	1.0	40.6
ycf1_p5	TGGTAATCAGTATCAGGAAGAAGG	TGAGGCTCTAGATATGGGATT	41.7	42.9	57.5	55.0	249 bp	1.0	40.6