Hotspot

rpl32-trnL-TAG

SSC · 116166-116961 · 796 bp

Back to Cheirodendron

Pi 0.00251

MarkerSeek score 60.20

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 796
variable_sites: 2
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	923-932 bp	1.0	78.8
rpl32-trnL-TAG_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	924-933 bp	1.0	78.8
rpl32-trnL-TAG_p3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	933-942 bp	1.0	77.8
rpl32-trnL-TAG_p4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	912-921 bp	1.0	77.6
rpl32-trnL-TAG_p5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	913-922 bp	1.0	77.6