Hotspot

ndhF-rpl32

SSC · 114775-116000 · 1226 bp

Back to Cheirodendron

Pi 0.00165

MarkerSeek score 59.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1226
variable_sites: 2
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1411-1412 bp	1.0	78.5
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1409-1410 bp	1.0	78.4
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1410-1411 bp	1.0	78.4
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1422-1423 bp	1.0	77.3
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	GACAACGCTTTTAAGGCTGC	45.0	50.0	58.0	58.3	1420-1421 bp	1.0	77.3